Adequacy SurePath 9, ThinPrep 4 Endocervical/transformation component Normal cell Superficial cell Pyknotic Keratohyaline granules (HMCK) Intermediate cells Finely granular chromatin Basic size reference Naked nuclei second half of cycle, bacterial cytolysis Parabasal cell Larger nucleus Granular dense cytoplasm higher N/C ratio Endocervical cells Cytoplasm diffusely vacuolated or granular Polarity, nucleus and mucus Endometrial cell Tight cluster Smaller nucleus Dense heterogeneous apoptotic debris Cytoplasm scant dense vacuolated 3 dimension Nonneoplastic variation Squamous metaplasia Variable N/C ratio Keratotic cellular change Keratohyaline granules in intermediate cells Typical parakeratosis Atrophy Thin, immature basal/parabasal cells only Flat monolayer sheet of parabasal-type cells Preserved nuclear polarity Streaming pattern Atrophic vaginitis, blue blobs Reactive cellular change associated with inflammation (Typical repair) Nulear enlargement Without chromatin change Prominant nucleolus Distinct cytoplasmic border RCC associated with Radiation Bizarre cells Polychromasia Nuclear degeneration, cytoplasmic vacuolization Organisms Trichomonas vaginalis Pear shape Nucleus pale, eccentric Eosinophilic cytoplasmic granules Flagella Fungus Candida Pseudohyphae, budding yeast Spearing, shish kebab Shift in flora s/o bacterial vaginosis Coccobacilli absence of lactobacilli Clue cell Actinomycosis Filamentous organism Acute angles Cotton ball Herpes simplex virus Nuclei ground glass Enhance nuclear envelope peripheral chromatin margination Dense eosinophilic intranuclear inclusion (Cowdry) 3Ms Multinucleation, molding, margination of chromatin Cytomegalovirus Large eosinophilic intranuclear viral inclusions with a prominent halo Small cytoplasmic basophilic inclusion
Dr. Dina R. Mody HPV test DNA HPV test will fail. BD (Oncolarity) is for a screening test. genotype 66 high risk. False-negative 10%. DNA copy number is the cause. False positive cross reactivity 0.35%, 0.71% Aptima and Cobas. (one quarter) Dr. Sneige Immunostain in breast Reduced and diminished myoepithelial cells, phenotypic alteration, SMA remain but others lost. Any positivity for MEC favors in situ than invasive. Metaplastic carcinoma and fibromatosis like TTF-1 2% positive in breast cancer.